SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma
DSMZ catalog code: ACC 106
Morphology: adherent cells growing in big clusters, different cellular forms
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: rat hepatoma a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma ( 7795, Buffalo strain) in 1967; cells were described to synthesize and to secrete serum albumin and serum complement component C9 confirmed as rat with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. M. Ferro, Institute of General Pathology, University of Genova, Genova, Italy
General Restrictions
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Dulbecco's MEM + 10-15% FBS split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm 2 at 37C with 5-10% CO2 cell harvest of about 20 x 106 cells/175 cm 2;
doubling time of ca. 33-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 10% polyploidy; 44(40-46)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of MPI, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 144, ECACC: 85112702, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma
ECACC catalog code: 85112702
Morphology: Epithelial, compact smooth bordered colonies
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: Rat liver hepatoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Rat serum albumin, tyrosine aminotransferase Applications: Hormone and Enzymatic studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) or Ham's F10 + 10% Horse Serum (HS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No. 48; hyperdiploid
Derived from the Morris Hepatoma but is distinct from HTC. Immunological experiments demonstrate that the cultures synthesise and secrete rat serum albumin and synthesise tyrosine aminotransferase. Functions of the cells include the conjugation and metabolism of testosterone and bilirubin and the production of serum complement component, especially C9.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 144, DSMZ: ACC 106, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
ICLC catalog code: ATL98014
Continuous culture, grown as monolayer
epithelial; the cells grow in large clusters morphology
SPECIES: rat
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells synthesize and secrete serum albumin, C9, tyrosine aminotransferase; monooxigenases expression; toxicity screening; xenobiotic metabolism; drug metabolism assays; hepatocyte studies
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
cell harvest is about 10 x 10^6 cells/25 cm2 flask
Validated by: isoenzymes (confirmed as rat with AST, MD)
Availability in cell line catalogues: ECACC: 85112702, DSMZ: ACC 106
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- J Cell Biol 1969;40:236-247 - PMID: 4177659
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
|
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
|
