MG-63's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MG-63 (human, osteosarcoma)

ICLC catalog code: HTL99003
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 years old)
TUMOR: osteosarcoma
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce high levels of interferon following superinduction with polyinosinic acid-polycytidilic acid, cycloheximide and actinomycin D

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: IZSBS: BS TCL40, ECACC: 86051601

Bibliographic references:

Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813

MG-63 (human, bone, osteosarcoma)

IZSBS catalog code: BS TCL40
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 year old) Karyology: hypotriploid
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
PROPERTIES: interferon prod.; interferon induction

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% inactivated FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 96 , mycoplasma negative, culture
Producing high yields of interferon; antigenically MG63 interferon is said to be more closely related to human fibroblast than to leukocyte interferon.

Availability in cell line catalogues: ATCC: CRL 1427, ECACC: 86051601, ICLC: HTL99003

MG-63 (human, bone, osteosarcoma)

Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 year old) Karyology: hypotriploid
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
PROPERTIES: interferon prod.; interferon induction

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2, split 1-5

Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003

Bibliographic references:

Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813

MG-63 (human, bone, osteosarcoma)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003

Bibliographic references:

Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105