SPECIES: human
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
ECACC catalog code: 86051601
Description: Human osteosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Inducible interferon Applications: Interferon-induction studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 107;
Karyotype: Modal No. 66; hypotriploid
From osteosarcoma of 14 year male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ICLC: HTL99003
Bibliographic references:
- Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813
ICLC catalog code: HTL99003
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 years old)
TUMOR: osteosarcoma
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce high levels of interferon following superinduction with polyinosinic acid-polycytidilic acid, cycloheximide and actinomycin D
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: IZSBS: BS TCL40, ECACC: 86051601
Bibliographic references:
- Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 year old) Karyology: hypotriploid
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
PROPERTIES: interferon prod.; interferon induction
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003
Bibliographic references:
- Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: human (male, 14 year old) Karyology: hypotriploid
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
PROPERTIES: interferon prod.; interferon induction
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003
Bibliographic references:
- Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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