TISSUE/ORGAN: bone marrow
TUMOR: leukemia, megakaryoblastic
ECACC catalog code: 94012401
Description: Human megakaryoblastic leukaemia
Viruses: See Properties
Depositor: Dr M Takeuchi, Institute for Fermentation, Osaka, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG)-Fc in 12% of cells; C3' in 53% of cells Applications: Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x100,000 by pipetting without the use of trypsin/EDTA. Allow cells to settle, pipette into fresh media. Saturation density is reached at 4x1,000,000; 5% CO2; 37C.
Karyotype: Modal No 56-58, hyperdiploid
Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species ocnfirmation)
Availability in cell line catalogues: ATCC: CRL 2021, ICLC: HTL96017, DSMZ: ACC 364
- Blood 1985;66:1384-1392 - PMID: 2998511
- Exp Cell Res 1991;193:223 - PMID: 1995298
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.