SPECIES: bovine
TISSUE/ORGAN: kidney
DSMZ catalog code: ACC 174
Morphology: adherent, epithelial-like cells
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis
Description: bovine kidney cells cells were derived from a kidney of a normal adult steer in 1957; cells are BVD-free (bovine diarrhea virus) confirmed as bovine with IEF of AST, G6PD, NP
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institut, BFA, Insel Riems, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence; seed out 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 5-10 x 106 cells/80 cm 2;
doubling time of ca. 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as bovine with IEF of AST, G6PD, NP)
Availability in cell line catalogues: ATCC: CRL 6071, IZSBS: BS CL63, ECACC: 90050801
Bibliographic references:
- Proc Soc Exp Biol Med 1958;98:574
SPECIES: bovine
TISSUE/ORGAN: kidney
ECACC catalog code: 90050801
Morphology: Epithelial-like
Description: Bovine kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Bos virus
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (BVDV negative) or 10% Horse Serum (HS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 120;
Karyotype: Modal No. 51; hypodiploid
Derived from the kidney of an apparently normal, adult Bos taurus. These cells are BVDV-free. Also known as MDBK (NBL-1).
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 22, IZSBS: BS CL63, DSMZ: ACC 174
Bibliographic references:
- J Natl Cancer Inst 1986;76:87-93 - PMID: 3455746
- Proc Soc Exp Biol Med 1958;98:574
IZSBS catalog code: BS CL63
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: bovine
TISSUE/ORGAN: kidney
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 6071, DSMZ: ACC 174, ECACC: 90050801
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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