MDA-MB-231's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 51 years old) Karyology: mean chromosomal number 68
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: specialized product secr.; estrogen receptor negative; chemotherapeutic agents; hormone sensibility; cell biology; chemosensibility

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIAP, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 32
Derived from pleural effusion

Availability in cell line catalogues: ATCC: HTB 26, ECACC: 92020424, ICLC: HTL99004

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

ICLC catalog code: HTL99004
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 51 years old)
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: negative for estrogen receptors (ER-)

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: ECACC: 92020424

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 51 years old) Karyology: mean chromosomal number 68
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: specialized product secr.; estrogen receptor negative; chemotherapeutic agents; hormone sensibility; cell biology; chemosensibility

Available in the following LABORATORY:

Dip. Scienze Biomediche ed Oncologia Umana (TOUCI, Torino)
L-15 + 10% FBS; Freezing medium: Culture medium + 5% or 10% DMSO; 37C, 5% CO2, split 1-2 Passage: 28

Availability in cell line catalogues: ATCC: HTB 26, ECACC: 92020424, ICLC: HTL99004

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105