SPECIES: human, Caucasian
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
ECACC catalog code: 86012803
Morphology: Epithelial-like
Endogenous viruses: contains type B * contains type C
Description: Human Caucasian breast adenocarcinoma
Viruses: See Properties
Depositor: Dr M Shaw, ICI Pharmaceuticals Ltd, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Reported to express the oestrogen and progesterone receptor Applications: Tumourigenicity and virus studies: Type B and C viruses
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, hypertriploid to hypotetraploid
Established from pleural effusion from 69 year female Caucasian. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of 'domes'. Cells may carry B or C type retrovirus and is considered to represent a category 2 pathogen (P2 containment). Cells express both the wildtype and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 22, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
ICLC catalog code: HTL95021
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: estrogen receptor positive; specialized product secretion; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
The viability may be low after thawing; thaw with medium supplemented with 20% FBS
Validated by: isoenzymes (confirmed as human with G6PD, NP)
Availability in cell line catalogues: ECACC: 86012803
Bibliographic references:
- Cancer Res 1990;118:48 - PMID: 2152773
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
-
Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-5 Passage: 143 , mycoplasma negative
Validated by: isoenzymes (PGM3,1; PGM1,1-2; ES D,1-2; AK1,1; GLO-1,1-2; G6PD,B)
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
-
Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
The ability to process estradiol via cytoplastic estrogen receptors and to form domes.
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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