MCF7's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MCF7 (human, Caucasian, breast, adenocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIAP, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 152

Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021

Bibliographic references:

J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

MCF7 (human, Caucasian, breast, adenocarcinoma)

ICLC catalog code: HTL95021
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: estrogen receptor positive; specialized product secretion; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
The viability may be low after thawing; thaw with medium supplemented with 20% FBS
Validated by: isoenzymes (confirmed as human with G6PD, NP)

Availability in cell line catalogues: ECACC: 86012803

Bibliographic references:

Cancer Res 1990;118:48 - PMID: 2152773
J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

MCF7 (human, Caucasian, breast, adenocarcinoma)

Available in the following LABORATORY:

Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-5 Passage: 143 , mycoplasma negative
Validated by: isoenzymes (PGM3,1; PGM1,1-2; ES D,1-2; AK1,1; GLO-1,1-2; G6PD,B)

Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021

Bibliographic references:

J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

MCF7 (human, Caucasian, breast, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021

Bibliographic references:

J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

MCF7 (human, Caucasian, breast, adenocarcinoma)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
The ability to process estradiol via cytoplastic estrogen receptors and to form domes.

Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021

Bibliographic references:

J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

MCF7 (human, Caucasian, breast, adenocarcinoma)

Available in the following LABORATORY:

Dip. Scienze Biomediche ed Oncologia Umana (TOUCI, Torino)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 5% or 10% DMSO; 37C, 5% CO2, split 1-2 Passage: 85

Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021

Bibliographic references:

J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105