McA-RH7777's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

McA-RH7777 (rat, buffalo, liver, hepatoma)

ICLC catalog code: ATL00002
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: albumin and alpha fetoprotein production

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + Ham's F12 (1:1) + 7-10% FBS + 2 mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR

Validated by: isoenzymes (confirmed as rat with MD, NP)

Bibliographic references:

Onco-Developmental Gene Expression, pp. 259-270, Fishman and Sell eds., Academic Press, New York, 1976
Recent Results Cancer Res 1974;44:103-114

McA-RH7777 (rat, buffalo, liver, hepatoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, buffalo (female)
TISSUE/ORGAN: liver
TUMOR: hepatoma
tumorigenic and metastatic in syngeneic animals
PROPERTIES: albumin prod.; fetoprotein alpha prod.; steroid hormones sensitive; tumorigenicity; toxicity screening; cytotoxicity; xenobiotic metabolism

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1601, ICLC: ATL00002

Bibliographic references:

J Biol Chem 1982;257:5827 - PMID: 6175632
Onco-Developmental Gene Expression (Fishman and Sell ed, Academic Press N Y) 1976;259-270
Recent Results Cancer Res 1974;44:103

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105