SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
ECACC catalog code: 87060101
Morphology: Epithelial
Description: Human colon adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 44;
Karyotype: Modal No. 49, (2n=46)
Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA)..
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 229, ICLC: HTL99028, DSMZ: ACC 350
Bibliographic references:
- Cancer Res 1979;39:2630-2636 - PMID: 445465
- Exp Cell Res 1976;101:414-416 - PMID: 964319
- J Natl Cancer Inst 1978;61:75-83 - PMID: 276641
ICLC catalog code: HTL99028
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 56 years old)
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production. HLA: A11; B15, B17; Cw1, Cw3.
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:2-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 87060101, DSMZ: ACC 350
Bibliographic references:
- Cancer Res 1976;36:467-475 - PMID: 1260746
- Cancer Res 1979;39:2630-2636 - PMID: 445465
- Exp Cell Res 1976;101:414-416 - PMID: 964319
- J Natl Cancer Inst 1978;61:75-83 - PMID: 276641
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 56 years old)
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: CEA prod.; tumor markers; pharmacodynamics; chemotherapeutic agents; antitumour testing; cell biology; cytotoxicity; cloning; tumorigenicity; drug resistance
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 229, ICLC: HTL99028, ECACC: 87060101, DSMZ: ACC 350
Bibliographic references:
- Cancer Res 1979;39:2630-2636 - PMID: 445465
- Exp Cell Res 1976;101:414-416 - PMID: 964319
- J Natl Cancer Inst 1978;61:75-83 - PMID: 276641
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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