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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

L1210 (mouse, DBA/2, blood, leukemia, lymphocytic)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  Fischer's + 2mM Glutamine + 10% Horse Serum (HS) or ATCC CRCM30 + 10% Horse Serum (HS). Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO2; 37C.
  Passages: 380; Karyotype: 2n = 40, diploid
  Established from a tumour induced in an 8 month old female 234 sub-line 212 of a DBA mouse. Originally sub-cultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 219, ICLC: ATL99002

Bibliographic references:

• J Natl Cancer Inst 1949;10:179-192
  
• J Natl Cancer Inst 1966;36:405-421
  

L1210 (mouse, DBA/2, ascitic fluid, leukemia, lymphocytic)

Available in the following LABORATORY:

• IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
  McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 1-5 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 Passage: 285 , mycoplasma negative, HOECHST and PCR
  Validated by: isoenzymes (confirmed as mouse with MD, NP)
  

Availability in cell line catalogues: ECACC: 87092804

Bibliographic references:

• J Natl Cancer Inst 1949;10:179-192

L1210 (mouse, DBA/2, ascitic fluid, leukemia, lymphocytic)

Available in the following LABORATORY:

• Istituto Ricerche Farmacologiche Mario Negri (MIILC, Milano)
  RPMI 1640 + 10% FBS + 2mM L-Glutamine + Hepes buffer + 2-mercaptoethanol; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
  Doubling time 12 h. Seeding concentration 1 x 105 cells/ml; split when 6 x 105 cells/ml. Every 2-3 weeks centrifuge and resuspend in fresh medium. Tumor induced in vivo by methylcholanthrene.
  

Availability in cell line catalogues: ATCC: CCL 219, ECACC: 87092804, ICLC: ATL99002

Bibliographic references:

• J Natl Cancer Inst 1949;10:179-192
• J Natl Cancer Inst 1966;36:405-421

L1210 (mouse, DBA/2, ascitic fluid, leukemia, lymphocytic)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  
  

Availability in cell line catalogues: ATCC: CCL 219, ECACC: 87092804, ICLC: ATL99002

Bibliographic references:

• J Natl Cancer Inst 1949;10:179-192
• J Natl Cancer Inst 1966;36:405-421