SPECIES: human
TISSUE/ORGAN: bone marrow
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 14
Morphology: round, single cells in suspension
Description: human acute myeloid leukemia established from the bone marrow of a 59-year-old man with erythroleukemia that developped into acute myeloid leukemia (AML) at relapse in 1977; cells were described to form colonies in soft-agar in response to colony-stimulating factor confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at 1.0-1.5 x 106 cells/ml;
doubling time of ca. 38 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 5% polyploidy; 45(42-47)<2n>X, -Y, -7, -12, +2m, dup(7)(q12q33), der(8)t(8;?)(p12;?), der(11)t(1;11)(q11;p11), der(17)t(17;?)(p13;?); does not correspond clearly with original karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 246, ECACC: 86111306
Bibliographic references:
- Blood 1980;56:344-350 - PMID: 6996765
- Blood 1983;62:709-721 - PMID: 6192859
- Science 1978;200:1153-1154 - PMID: 306682
SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: leukemia, acute myeloid
ECACC catalog code: 86111306
Morphology: Myeloblast like
Description: Human Caucasian bone marrow myelogenous leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 46, diploid
Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 246, DSMZ: ACC 14
Bibliographic references:
- Blood 1980;56:344-350 - PMID: 6996765
- Blood 1983;62:709-721 - PMID: 6192859
- Science 1978;200:1153-1154 - PMID: 306682
Continuous culture, grown as suspension
myeloblast-like morphology
SPECIES: human, Caucasian (male, 59 years old)
TISSUE/ORGAN: bone marrow
TUMOR: leukemia, acute myeloid
non tumorigenic
PROPERTIES: tumor markers; tumorigenicity; differentiation; cloning; CSF, responsive to; DR antigen expression; Ia-like antigen expression
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 246, ECACC: 86111306, DSMZ: ACC 14
Bibliographic references:
- Blood 1980;56:344-350 - PMID: 6996765
- Blood 1983;62:709-721 - PMID: 6192859
- Science 1978;200:1153-1154 - PMID: 306682
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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