SPECIES: human, Caucasian
TISSUE/ORGAN: mouth
TUMOR: carcinoma, epidermoid
ECACC catalog code: 94050408
Morphology: Epithelial-like
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
Description: Human oral epidermoid carcinoma, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: KB has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumourigenicity and viruses e.g. susceptible to Polio virus type I and Adenovirus type 3.
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 370;
Karyotype: 2n = 46
Derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, DSMZ: ACC 136
Bibliographic references:
- Arch Virol 1980;66:309 - PMID: 7447707
- In Vitro 1978;14:469-475 - PMID: 566722
- Nature 1976;259:211-213 - PMID: 1250349
- Proc Soc Exp Biol Med 1955;89:362
- Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453
IZSBS catalog code: BS TCL34
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 17, ECACC: 94050408, DSMZ: ACC 136
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408, DSMZ: ACC 136
Bibliographic references:
- Arch Virol 1980;66:309 - PMID: 7447707
- In Vitro 1978;14:469-475 - PMID: 566722
- Nature 1976;259:211-213 - PMID: 1250349
- Proc Soc Exp Biol Med 1955;89:362
- Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology
Available in the following LABORATORY:
-
Universita' degli Studi (PRUMB, Parma)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 400
Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408, DSMZ: ACC 136
Bibliographic references:
- Arch Virol 1980;66:309 - PMID: 7447707
- In Vitro 1978;14:469-475 - PMID: 566722
- Nature 1976;259:211-213 - PMID: 1250349
- Proc Soc Exp Biol Med 1955;89:362
- Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408, DSMZ: ACC 136
Bibliographic references:
- Arch Virol 1980;66:309 - PMID: 7447707
- In Vitro 1978;14:469-475 - PMID: 566722
- Nature 1976;259:211-213 - PMID: 1250349
- Proc Soc Exp Biol Med 1955;89:362
- Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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