KB's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

KB (human, Caucasian, cervix, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: cervix
TUMOR: carcinoma

DSMZ catalog code: ACC 136
Morphology: adherent cells, epithelial-like, growing as monolayers
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
Description: human cervix carcinoma (derivative of HELA) originally described as 'epidermoid carcinoma established from the mouth of a Caucasian man in 1954'; however, as shown by DSMZ DNA fingerprinting (see below) this cell line must be considered an HELA subclone (human cervix carcinoma, see HELA DSM ACC 57) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + MEM non-essential amino acids + 0.075% Na-bicarbonate split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA at 37C with 5% CO2 about 5-10 x 106 cells/80 cm 2 flask; frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 8% polyploidy; 76(73-78)<3n>) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as HELA) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin++ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408

Bibliographic references:

Arch Virol 1980;66:309 - PMID: 7447707
In Vitro 1978;14:469-475 - PMID: 566722
Nature 1976;259:211-213 - PMID: 1250349
Proc Soc Exp Biol Med 1955;89:362
Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453

KB (human, Caucasian, mouth, carcinoma, epidermoid)

SPECIES: human, Caucasian
TISSUE/ORGAN: mouth
TUMOR: carcinoma, epidermoid

ECACC catalog code: 94050408
Morphology: Epithelial-like
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
Description: Human oral epidermoid carcinoma, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: KB has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumourigenicity and viruses e.g. susceptible to Polio virus type I and Adenovirus type 3.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 370; Karyotype: 2n = 46
Derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, DSMZ: ACC 136

Bibliographic references:

Arch Virol 1980;66:309 - PMID: 7447707
In Vitro 1978;14:469-475 - PMID: 566722
Nature 1976;259:211-213 - PMID: 1250349
Proc Soc Exp Biol Med 1955;89:362
Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453

KB (human, Caucasian, mouth, carcinoma, squamous cell)

IZSBS catalog code: BS TCL34
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 375 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CCL 17, ECACC: 94050408, DSMZ: ACC 136

KB (human, Caucasian, mouth, carcinoma, squamous cell)

Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, Adult) Karyology: modal number 74-78
TISSUE/ORGAN: mouth
TUMOR: carcinoma, squamous cell
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
PROPERTIES: nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma positive
HeLa markers. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408, DSMZ: ACC 136

Bibliographic references:

Arch Virol 1980;66:309 - PMID: 7447707
In Vitro 1978;14:469-475 - PMID: 566722
Nature 1976;259:211-213 - PMID: 1250349
Proc Soc Exp Biol Med 1955;89:362
Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453

KB (human, Caucasian, mouth, carcinoma, squamous cell)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

HeLa markers.

Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408, DSMZ: ACC 136

Bibliographic references:

Arch Virol 1980;66:309 - PMID: 7447707
In Vitro 1978;14:469-475 - PMID: 566722
Nature 1976;259:211-213 - PMID: 1250349
Proc Soc Exp Biol Med 1955;89:362
Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105