KARPAS-422's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

KARPAS-422 (human, lymphoma, follicular, B cell)

ICLC catalog code: HTL99023
Continuous culture, grown as suspension
round to polygonal cells, growing in single or in small clusters morphology
SPECIES: human (female, 73 years old) Karyology: (14;18)(q32,q21) chromosomal translocation
TUMOR: lymphoma, follicular, B cell
Endogenous viruses: contains Epstein-Barr
DEPOSITOR: Dr G. Canti, Pharmacology Dept, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: IgH/bcl2 fusion gene; CD3- CD10+ CD13(+) CD19+ CD20+ CD34+ CD37+ EBV+

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
the cells are difficult to culture; viability may be low after thawing
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: DSMZ: ACC 32

Bibliographic references:

Blood 1990;75:709-714 - PMID: 2297573

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105