K-562's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Continuous culture, grown as suspension
erythromyeloblastoid morphology
SPECIES: human, Caucasian (female, 53 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, chronic myeloid
tumorigenic in nude mice
PROPERTIES: haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cell biology; cellular effects of hyperthermia; natural killer assay

Available in the following LABORATORY:

Istituto Nazionale Riposo e Cura Anziani (INRCA) (ANIIR, Ancona)
mycoplasma negative
Validated by: isoenzymes (AK1,1; ESD,1; GLO-1,2; GGPD,B; PPM1,0; PGM3,1; ME-2,0)

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Monoclonal continuous culture, grown as suspension
erythromyeloblastoid morphology
SPECIES: human, Caucasian (female, 53 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, chronic myeloid
tumorigenic in nude mice
PROPERTIES: haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cell biology; cellular effects of hyperthermia; natural killer assay

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-6 , mycoplasma negative, DAPI and HOECHST
* Split twice a week
Validated by: isoenzymes

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Continuous culture, grown as suspension
erythromyeloblastoid morphology
SPECIES: human, Caucasian (female, 53 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, chronic myeloid
tumorigenic in nude mice
Cell line available under some conditions.
PROPERTIES: haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cell biology; cellular effects of hyperthermia; natural killer assay

Available in the following LABORATORY:

Dipartimento Oncologia e Neuroscienze, Universita'degli Studi (CHUIM, Chieti)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, leukemia, chronic myeloid)

SPECIES: human
TUMOR: leukemia, chronic myeloid

DSMZ catalog code: ACC 10
Morphology: round large, single cells in suspension
Description: human chronic myeloid leukemia in blast crisis established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; cells can be used as highly sensitive targets in in-vitro natural killer assays; cells produce hemoglobin; cells carry the Philadelphia chromosome with a b3-a2 fusion gene confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 3 days; seed out at ca. 0.3-0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at 1.0-1.5 x 106 cells/ml; doubling time of ca. 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypotriploid without sharp mode; 61-68<3n>XX, -X, -3, +7, -13, -18, +3mar, del(9)(p11-13), der(14)t(14;?)(p11;?), der(17)t(17;?)(p11-13;?), der(?18)t(15;?18)(q21;?q12), del(X)(p22); two markers appear from FISH to have arisen from h) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD19- glycophorin A+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 243, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, myelogenous)

ICLC catalog code: HTL94001
Continuous culture, grown as suspension
round and large cells morphology
SPECIES: human, Caucasian (female, 53 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, myelogenous
tumorigenic in nude mice
DEPOSITOR: Dr V. Dessi, IRCCS Ospedale Policlinico San Martino, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cellular effects of hyperthermia; natural killer assay

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 1 and 5x105 cells/ml; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with AST, NP, Pep B)

Availability in cell line catalogues: DSMZ: ACC 10

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2 , mycoplasma negative
Validated by: isoenzymes (AK1,1; ES D,1; GLO-1,2; G6PD,B; PGM1,0; PGM3,1; Me-2,0)

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

ENEA, CRE-Casaccia (RMEBA, Roma)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-9 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

K-562 (human, Caucasian, pleural effusion, leukemia, chronic myeloid)

Available in the following LABORATORY:

Dip.Medicina Sperimentale e Scienze Biochimiche, Universita' degli Studi (RMUFM, Roma)
RPMI 1640 + 10% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Validated by: PDGF/2 production

Availability in cell line catalogues: ATCC: CCL 243, DSMZ: ACC 10, ICLC: HTL94001

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1973;50:535-538 - PMID: 4512889

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105