ECACC catalog code: 90111913
Susceptibility to viruses: vesic. stomatitis (Indiana)
Description: Rat sarcoma, asparagine requiring
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Metabolic and cloning studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Renew medium daily where possible. Subculture by placing culture in cold room (4C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and into fresh flasks. Split confluent cultures 1:2 to 1:10; 5% CO2; 37C.
Karyotype: 2n = 42, hyperdiploid
Used in studies relating to amino acid requirements and L-asparagine metabolism. Cloning studies show that variants not requiring L-asp are present in 100 per 1,000,000 cells Cloned populations which do not produce variants immediately do so after 6-8 further passages.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 45, IZSBS: BS TCL 31
- Biochem Biophys Res Commun 1967;26:228 - PMID: 6067662
- Cancer Res 1959;19:591
- Proc Soc Exp Biol Med 1969;131:131 - PMID: 5770086
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.