ICLC catalog code: ATL98011
Continuous culture, grown as monolayer
macrophage-like, many round cells morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: monocyte-macrophage
TUMOR: monocyte-macrophage tumor
DEPOSITOR: Dr A. Corsini, Institute of Pharmacological Sciences, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells have been described to synthesize lysozyme and IL-1, and have receptors for Ig and complement
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA or scraper: the cells are very difficult to detach; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: ECACC: 91051511
Bibliographic references:
- Cancer Res 1977;37:546-550 - PMID: 318922
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1975;114:898-905 - PMID: 1089721
- Nature 1975;257:393-394 - PMID: 1101071
Monoclonal continuous culture, grown as monolayer
macrophage morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: monocyte-macrophage
TUMOR: not defined tumor
PROPERTIES: IL-1 prod.; lysozyme synthesis; complement receptor; Fc receptor; biochemistry; metabolism; Ig and complement receptor
Available in the following LABORATORY:
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Pharmacia Upjhon (MIECC, Nerviano (MI))
DMEM + 10% FBS + 1% non-essential amino acids + NaHCO3 (24mM) + Tricine (20mM); Freezing medium: DMEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 1-3 , mycoplasma negative
Availability in cell line catalogues: ATCC: TIB 67, ECACC: 91051511, ICLC: ATL98011
Bibliographic references:
- Cancer Res 1977;37:546-550 - PMID: 318922
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1975;114:898-905 - PMID: 1089721
- J Immunol 1977;119:950-954 - PMID: 894031
- Nature 1975;257:393-394 - PMID: 1101071
Continuous culture, grown as monolayer
macrophage morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: monocyte-macrophage
TUMOR: not defined tumor
PROPERTIES: IL-1 prod.; lysozyme synthesis; complement receptor; Fc receptor; biochemistry; metabolism; Ig and complement receptor
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: TIB 67, ECACC: 91051511, ICLC: ATL98011
Bibliographic references:
- Cancer Res 1977;37:546-550 - PMID: 318922
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1975;114:898-905 - PMID: 1089721
- J Immunol 1977;119:950-954 - PMID: 894031
- Nature 1975;257:393-394 - PMID: 1101071
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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