Monoclonal continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
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Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% Antibiotics + 1% non-essential amino acids; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-2 , mycoplasma negative, DAPI and HOECHST
Validated by: immunofluorescence * isoenzymes * membrane markers
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro (GEICV, Genova)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2
Undifferentiated morphology. They will differentiate into neuron with retinoic acid
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
ICLC catalog code: HTL96021
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
The viability can be low after thawing; thaw with medium supplemented with 20% FBS.
Validated by: isoenzymes (confirmed as human with AST, MD)
Availability in cell line catalogues: DSMZ: ACC 165
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
(reference paper)
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Monoclonal continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Monoclonal continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
-
Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-3 , mycoplasma negative
Grows also as clumps in suspension
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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