SPECIES: human, Caucasian
TUMOR: neuroblastoma
DSMZ catalog code: ACC 165
Morphology: fibroblast-like adherent cells growing as monolayer
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
Description: human neuroblastoma established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS + 1x non-essential amino acids split confluent culture 1:2 every 3-5 days (trypsin should not be used, cells can be detached by tapping the flask or pipetting); seed out at ca. 0.5-1.0 x 106 cells/cm 2 at 37C with 5% CO2 about 1-2 x 106 cells/ml or 0.5 x 106 cells/cm 2;
doubling time of ca. 40-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1;2HSR)(p33/35;?p22)x2, der(16)t(2;16)(p21/22;q24/25); HSR regions probably represent amplification of the N-myc oncogene which maps to 2p22 involved in the t(2;16) als) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament+ vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 127, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro (GEICV, Genova)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2
Undifferentiated morphology. They will differentiate into neuron with retinoic acid
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
ICLC catalog code: HTL96021
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
The viability can be low after thawing; thaw with medium supplemented with 20% FBS.
Validated by: isoenzymes (confirmed as human with AST, MD)
Availability in cell line catalogues: DSMZ: ACC 165
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
(reference paper)
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Monoclonal continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Monoclonal continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
-
Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-3 , mycoplasma negative
Grows also as clumps in suspension
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
Continuous culture, grown as monolayer
fibroblast-like and neuroblast-like morphology
SPECIES: human, Caucasian (male, 13 months old)
TUMOR: neuroblastoma
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: antigen presentation; biochemical markers; tumor markers; neurotransmitter prod.; chemotherapeutic agents; nucleic acids; receptor; tumorigenicity; differentiation; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 127, DSMZ: ACC 165, ICLC: HTL96021
Bibliographic references:
- Cancer Res 1970;30:2110 - PMID: 5459762
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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