ICLC catalog code: HTL99009
Continuous culture, grown as suspension
lymphoblast-like, single and clumped cells morphology
SPECIES: human (female)
TISSUE/ORGAN: bone marrow
DEPOSITOR: Dr D. Lattuada, Dept of Pharmacology, Chemotherapy and Medical Toxicology, Milan, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells express surface markers and receptors characteristics of B-lymphoblasts, are capable of synthesizing immunoglobulin and display receptors for human growth hormone, insulin and calcitonin
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 2-9 x 10^5 cells/ml; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 117, IZSBS: BS TCL 98
Bibliographic references:
- Ann N Y Acad Sci 1972, 190:221-234
- Nature 1974, 251:443-444
IZSBS catalog code: BS TCL 98
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human, Caucasian (female) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: bone marrow
TUMOR: multiple myeloma
DEPOSITOR: obtained from ATCC
PROPERTIES: This cell line has been used extensively in biosynthetic and surface marker studies presenting receptor sites for human growth hormone, insulin, calcitonin; Ig prod.
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, MPI, PEPB, AST, LD)
Availability in cell line catalogues: ATCC: CCL-159, DSMZ: ACC 117, ICLC: HTL99009
Bibliographic references:
- Nature 1974;251:443-444 - PMID: 4608784
- Proc Natl Acad Sci U S A 1974;71:84-88 - PMID: 4359334
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female)
TISSUE/ORGAN: bone marrow
TUMOR: multiple myeloma
PROPERTIES: IgG kappa prod.; growth hormone receptor, human; insulin receptor; calcitonin receptor; tumorigenicity; immunofluorescence; hormone studies; bcl-2 mRNA expression
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 159, DSMZ: ACC 117, ICLC: HTL99009, IZSBS: BS TCL 98
Bibliographic references:
- Ann N Y Acad Sci 1972;190:221-234
- J Biol Chem 1974;249:1661-1667 - PMID: 4361817
- Nature 1974;251:443-444 - PMID: 4608784
- Proc Natl Acad Sci U S A 1974;71:84-88 - PMID: 4359334
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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