SPECIES: human
TISSUE/ORGAN: lymphoblastoid B cell
TUMOR: multiple myeloma
DSMZ catalog code: ACC 117
Morphology: lymphoblastoid, single or clumped cells in suspension
Description: human B-lymphoblastoid cells (from patient with multiple myeloma) established from the bone marrow of a woman with multiple myeloma in 1967; cells were described to synthesize IgG and to have receptors for insulin and calcitonin; cells express bcl-2 mRNA; cells appear to be rather 'EBV+ B-lymphoblastoid cells' than myeloma-derived cells (see also Pellat-Deceunynk et al., Blood 86: 4001-4002, 1995) confirmed as human with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (CCL 159), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 1.0-1.75 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml, do not culture below 0.5 x 10 6 cells/ml; as the medium becomes quickly acidic (yellow) without a corresponding cell growth, it is advisable to change the supernatant medium daily at 37C with 5% CO2 maximal cell density of 2-3 x 106 cells/ml;
doubling time of about 45 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid; 46(44-46)<2n>XX; apparently normal karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD19+ CD37- HLA-DR+ cyIgG+ cy_- cyIgM- cy_+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP, Pep B) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ATCC: CCL 159, ICLC: HTL99009, IZSBS: BS TCL 98
Bibliographic references:
- Ann N Y Acad Sci 1972;190:221-234
- J Biol Chem 1974;249:1661-1667 - PMID: 4361817
- Nature 1974;251:443-444 - PMID: 4608784
- Proc Natl Acad Sci U S A 1974;71:84-88 - PMID: 4359334
ICLC catalog code: HTL99009
Continuous culture, grown as suspension
lymphoblast-like, single and clumped cells morphology
SPECIES: human (female)
TISSUE/ORGAN: bone marrow
DEPOSITOR: Dr D. Lattuada, Dept of Pharmacology, Chemotherapy and Medical Toxicology, Milan, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells express surface markers and receptors characteristics of B-lymphoblasts, are capable of synthesizing immunoglobulin and display receptors for human growth hormone, insulin and calcitonin
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 2-9 x 10^5 cells/ml; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 117, IZSBS: BS TCL 98
Bibliographic references:
- Ann N Y Acad Sci 1972, 190:221-234
- Nature 1974, 251:443-444
IZSBS catalog code: BS TCL 98
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human, Caucasian (female) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: bone marrow
TUMOR: multiple myeloma
DEPOSITOR: obtained from ATCC
PROPERTIES: This cell line has been used extensively in biosynthetic and surface marker studies presenting receptor sites for human growth hormone, insulin, calcitonin; Ig prod.
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, MPI, PEPB, AST, LD)
Availability in cell line catalogues: ATCC: CCL-159, DSMZ: ACC 117, ICLC: HTL99009
Bibliographic references:
- Nature 1974;251:443-444 - PMID: 4608784
- Proc Natl Acad Sci U S A 1974;71:84-88 - PMID: 4359334
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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