AF-2 clone 9B5's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

AF-2 clone 9B5 (mouse, BALB/c, hybrid, B cells x myeloma, myeloma)

ICLC catalog code: HYL95001
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid, B cells x myeloma
TUMOR: myeloma
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: Hybridoma producing IgM monoclonal antibody specific for AF-2 protein.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10; resuscitate with medium containing 20% FCS, with a feeder layer of 3T3. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with G6PD, NP, MD)

Bibliographic references:

Exp Cell Res 1991;193:411-419 - PMID: 2004654
J Cell Physiol 1991;149:567-574 - PMID: 1744180

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105