ACHN's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

ACHN (human, Caucasian, kidney, adenocarcinoma)

ICLC catalog code: HTL99007
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 22 years old)
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: antiproliferative studies, using human interferons or interferon inducers

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR - mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: IZSBS: BS TCL68, ECACC: 88100508

Bibliographic references:

Cancer Res 1987;47:4582-4589 - PMID: 2957045

ACHN (human, Caucasian, kidney, adenocarcinoma)

IZSBS catalog code: BS TCL68
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male, 22 years old)
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: interferon assay; interferon induction

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 9 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CRL 1611, ECACC: 88100508, ICLC: HTL99007

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105