A549's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A549 (human, Caucasian, lung, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma

ECACC catalog code: 86012804
Morphology: Epithelial
Susceptibility to viruses: adenovirus
Description: Human Caucasian lung carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Lecithin, High amounts of de-saturated fatty acids. Applications: Enzymatic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12K or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 85; Karyotype: Hypotriploid
Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 185, ICLC: HTL03001, IZSBS: TCL 101

Bibliographic references:

Biochem J 1993;294(1):49-55 - PMID: 8395818
Biochem Soc Trans 1992;20(2):136S
Brain Res 1992;583(1-2):100-104 - PMID: 1324089
Carbohydr Res 1992;234:237-346 - PMID: 1334801
Eur J Pharmacol 1990;184(2-3):233-238 - PMID: 1964127
Eur J Pharmacol 1991;208(3):265-268 - PMID: 1685127
FASEB J 1993;7(14):1376-1380 - PMID: 8224610
FEBS Lett 1990;260(1):91-94 - PMID: 2153579
Int J Cancer 1976;17:62-70 - PMID: 175022
J Biol Chem 1990;265(36):22282-22287 - PMID: 2176212
J Biol Chem 1991;266(12):7876-7882 - PMID: 1902229
J Biol Chem 1991;266(13):8431-8438 - PMID: 2022658
J Biol Chem 1991;266(30):20519-20524 - PMID: 1657928
J Biol Chem 1993;268(26):19411-19415 - PMID: 8396137
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Neurochem 1991;57(3):963-969 - PMID: 1650398
J Neurochem 1993;61(2):12-17 - PMID: 8515256
J Pharmacol Exp Ther 1990;253(1):185-191 - PMID: 2329505
J Pharmacol Exp Ther 1993;264(3):1121-1127
Mol Cell Biol 1992;12(10):4742-4750 - PMID: 1328861
Mol Pharmacol 1990;37(2):296-303 - PMID: 1689454
Mol Pharmacol 1991;39(3):399-406 - PMID: 1706472
Mol Pharmacol 1993;44(2):364-370 - PMID: 8394993
Naunyn Schmiedebergs Arch Pharmacol 1992;346(1):51-56 - PMID: 1407005
Nippon Yakurigaku Zasshi 1992;99(1):1-11 - PMID: 1313776
Prostaglandins 1990;40(4):405-416 - PMID: 91126216
Symp Soc Exp Biol 1990;44:147-156 - PMID: 2130511

A549 (human, Caucasian, lung, carcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 58 years old)
TISSUE/ORGAN: lung
TUMOR: carcinoma
tumorigenic in athymic nude mice
Susceptibility to viruses: adenovirus
PROPERTIES: fatty acids synthesis; tumor markers; factor dependent; replication; enzymology; virology; lecithin synthesis

Available in the following LABORATORY:

Facolta' di Medicina e Chirurgia, Universita'degli studi (GEUEM, Genova)
Ham's F12 + 10% FBS + Antibiotics + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Ham's F12 M + 40% FBS + 10% DMSO; 37C, 5% CO2 Passage: 76 , mycoplasma negative
Surfactant action: pneumocytes type II. IGF-I factor dependent studies.

Availability in cell line catalogues: ATCC: CCL 185, ECACC: 86012804, ICLC: HTL03001, IZSBS: TCL 101

Bibliographic references:

Biochem J 1993;294(1):49-55 - PMID: 8395818
Biochem Soc Trans 1992;20(2):136S
Brain Res 1992;583(1-2):100-104 - PMID: 1324089
Carbohydr Res 1992;234:237-346 - PMID: 1334801
Eur J Pharmacol 1990;184(2-3):233-238 - PMID: 1964127
Eur J Pharmacol 1991;208(3):265-268 - PMID: 1685127
FASEB J 1993;7(14):1376-1380 - PMID: 8224610
FEBS Lett 1990;260(1):91-94 - PMID: 2153579
Int J Cancer 1976;17:62-70 - PMID: 175022
J Biol Chem 1990;265(36):22282-22287 - PMID: 2176212
J Biol Chem 1991;266(12):7876-7882 - PMID: 1902229
J Biol Chem 1991;266(13):8431-8438 - PMID: 2022658
J Biol Chem 1991;266(30):20519-20524 - PMID: 1657928
J Biol Chem 1993;268(26):19411-19415 - PMID: 8396137
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Neurochem 1991;57(3):963-969 - PMID: 1650398
J Neurochem 1993;61(2):12-17 - PMID: 8515256
J Pharmacol Exp Ther 1990;253(1):185-191 - PMID: 2329505
J Pharmacol Exp Ther 1993;264(3):1121-1127
Mol Cell Biol 1992;12(10):4742-4750 - PMID: 1328861
Mol Pharmacol 1990;37(2):296-303 - PMID: 1689454
Mol Pharmacol 1991;39(3):399-406 - PMID: 1706472
Mol Pharmacol 1993;44(2):364-370 - PMID: 8394993
Naunyn Schmiedebergs Arch Pharmacol 1992;346(1):51-56 - PMID: 1407005
Nippon Yakurigaku Zasshi 1992;99(1):1-11 - PMID: 1313776
Prostaglandins 1990;40(4):405-416 - PMID: 91126216
Symp Soc Exp Biol 1990;44:147-156 - PMID: 2130511

A549 (human, Caucasian, lung, carcinoma)

ICLC catalog code: HTL03001
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 58 year old)
TISSUE/ORGAN: lung
TUMOR: carcinoma
DEPOSITOR: Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells could synthesize lecithin and are positive for keratin

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)

Availability in cell line catalogues: ECACC: 86012804, IZSBS: TCL 101

Bibliographic references:

Int J Cancer 1976;17:62-70 - PMID: 175022
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A549 (human, lung, carcinoma)

IZSBS catalog code: TCL 101
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human Karyology: 2n = 46 in 40 cells
TISSUE/ORGAN: lung
TUMOR: carcinoma
DEPOSITOR: obtained from ATCC
PROPERTIES: producing lecithin; expressing xenobiotic metabolizing enzymes

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
Ham's F12K + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 77 , mycoplasma negative, culture
Expressing differentiated functions of alveolar type II cells
Validated by: isoenzymes (confirmed as human with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CCL-185, ECACC: 86012804, ICLC: HTL03001

Bibliographic references:

Int J Cancer 1976;17:62-70 - PMID: 175022
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A549 (human, Caucasian, lung, carcinoma)

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: CCL 185, ECACC: 86012804, ICLC: HTL03001, IZSBS: TCL 101

Bibliographic references:

Biochem J 1993;294(1):49-55 - PMID: 8395818
Biochem Soc Trans 1992;20(2):136S
Brain Res 1992;583(1-2):100-104 - PMID: 1324089
Carbohydr Res 1992;234:237-346 - PMID: 1334801
Eur J Pharmacol 1990;184(2-3):233-238 - PMID: 1964127
Eur J Pharmacol 1991;208(3):265-268 - PMID: 1685127
FASEB J 1993;7(14):1376-1380 - PMID: 8224610
FEBS Lett 1990;260(1):91-94 - PMID: 2153579
Int J Cancer 1976;17:62-70 - PMID: 175022
J Biol Chem 1990;265(36):22282-22287 - PMID: 2176212
J Biol Chem 1991;266(12):7876-7882 - PMID: 1902229
J Biol Chem 1991;266(13):8431-8438 - PMID: 2022658
J Biol Chem 1991;266(30):20519-20524 - PMID: 1657928
J Biol Chem 1993;268(26):19411-19415 - PMID: 8396137
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Neurochem 1991;57(3):963-969 - PMID: 1650398
J Neurochem 1993;61(2):12-17 - PMID: 8515256
J Pharmacol Exp Ther 1990;253(1):185-191 - PMID: 2329505
J Pharmacol Exp Ther 1993;264(3):1121-1127
Mol Cell Biol 1992;12(10):4742-4750 - PMID: 1328861
Mol Pharmacol 1990;37(2):296-303 - PMID: 1689454
Mol Pharmacol 1991;39(3):399-406 - PMID: 1706472
Mol Pharmacol 1993;44(2):364-370 - PMID: 8394993
Naunyn Schmiedebergs Arch Pharmacol 1992;346(1):51-56 - PMID: 1407005
Nippon Yakurigaku Zasshi 1992;99(1):1-11 - PMID: 1313776
Prostaglandins 1990;40(4):405-416 - PMID: 91126216
Symp Soc Exp Biol 1990;44:147-156 - PMID: 2130511

A549 (human, Caucasian, lung, carcinoma)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma positive
High activity of choline kinase and cholinephosphate cytidyl transferase. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CCL 185, ECACC: 86012804, ICLC: HTL03001, IZSBS: TCL 101

Bibliographic references:

Biochem J 1993;294(1):49-55 - PMID: 8395818
Biochem Soc Trans 1992;20(2):136S
Brain Res 1992;583(1-2):100-104 - PMID: 1324089
Carbohydr Res 1992;234:237-346 - PMID: 1334801
Eur J Pharmacol 1990;184(2-3):233-238 - PMID: 1964127
Eur J Pharmacol 1991;208(3):265-268 - PMID: 1685127
FASEB J 1993;7(14):1376-1380 - PMID: 8224610
FEBS Lett 1990;260(1):91-94 - PMID: 2153579
Int J Cancer 1976;17:62-70 - PMID: 175022
J Biol Chem 1990;265(36):22282-22287 - PMID: 2176212
J Biol Chem 1991;266(12):7876-7882 - PMID: 1902229
J Biol Chem 1991;266(13):8431-8438 - PMID: 2022658
J Biol Chem 1991;266(30):20519-20524 - PMID: 1657928
J Biol Chem 1993;268(26):19411-19415 - PMID: 8396137
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Neurochem 1991;57(3):963-969 - PMID: 1650398
J Neurochem 1993;61(2):12-17 - PMID: 8515256
J Pharmacol Exp Ther 1990;253(1):185-191 - PMID: 2329505
J Pharmacol Exp Ther 1993;264(3):1121-1127
Mol Cell Biol 1992;12(10):4742-4750 - PMID: 1328861
Mol Pharmacol 1990;37(2):296-303 - PMID: 1689454
Mol Pharmacol 1991;39(3):399-406 - PMID: 1706472
Mol Pharmacol 1993;44(2):364-370 - PMID: 8394993
Naunyn Schmiedebergs Arch Pharmacol 1992;346(1):51-56 - PMID: 1407005
Nippon Yakurigaku Zasshi 1992;99(1):1-11 - PMID: 1313776
Prostaglandins 1990;40(4):405-416 - PMID: 91126216
Symp Soc Exp Biol 1990;44:147-156 - PMID: 2130511

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105