HuP-T4's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HuP-T4 (human, Japanese, pancreas, carcinoma)

SPECIES: human, Japanese
TISSUE/ORGAN: pancreas
TUMOR: carcinoma

DSMZ catalog code: ACC 223
Morphology: epitheloid cells growing adherently in monolayers
Description: human pancreas carcinoma established from the malignant ascites of a 60-year-old Japanese man with pancreas carcinoma in 1985 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + 1% non-essential amino acids + 1% sodium pyruvate optimal split ratio of 1:2 to 1:3 every 2-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 1-2 x 105 cells/cm 2; doubling time of about 38 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm; 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, - 9, -9, +11, +16, +mar; inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12); 2 medium size ring ch prese t at 10%) * immunological analysis (cytokeratin + (100%), desmin -, endothel -, GFAP -, neurofilament -, vimentin + (1-5%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ECACC: 93121056, ICLC: HTL95006

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

HuP-T4 (human, pancreas, adenocarcinoma)

ICLC catalog code: HTL95006
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human (male, 60 year old) Karyology: modal number 77
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA and CA19-9 production

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
incubate with trypsin/EDTA at 37C for at least 10-15 min. After thawing the viability of the cells may be low.
Validated by: isoenzymes (confirmed as human with MD, MPI, NP)

Availability in cell line catalogues: ECACC: 93121056, DSMZ: ACC 223

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105