HuP-T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HuP-T3 (human, Japanese, pancreas, carcinoma)

SPECIES: human, Japanese
TISSUE/ORGAN: pancreas
TUMOR: carcinoma

DSMZ catalog code: ACC 259
Morphology: epitheloid cells growing adherently in monolayers and clusters
Description: human pancreas carcinoma established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984 confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 1% non-essential amino acids + 1% sodium pyruvate split confluent culture 1:10 once a week using trypsin/EDTA; seed out about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 about 14 x 106 cells/175 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.4 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hypodiploid karyotype with 13% polyploidy; 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3 mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12); gene amplification) * cytogenetics 2 (suggested by large HSR; ch17 nullisomy) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ECACC: 93121055, ICLC: HTL96006

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

HuP-T3 (human, Japanese, pancreas, adenocarcinoma)

ICLC catalog code: HTL96006
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Japanese (male, 66 years old) Karyology: modal chromosome number 37
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:10 using trypsin/EDTA(10-15 min); seed at 2-4x104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, NP)

Availability in cell line catalogues: ECACC: 93121055, DSMZ: ACC 259

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105