HTC's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HTC (rat, buffalo, liver, hepatoma)

SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma

ECACC catalog code: 93120108
Morphology: Epithelial
Description: Rat hepatoma
Viruses: See Properties
Depositor: Dr M Simpkins/Dr H Harris, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Dexamethasone phosphate; hydrocortisone Applications: Tumourigenicity and enzymatic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 9; Karyotype: 2n = 66, hypertriploid
Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N'-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
Research council deposit: Yes; Price Code: B;
Validated by: DNA fingerprinting * isoenzymes ((species confirmation))

Availability in cell line catalogues: ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

Monoclonal continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
Cell line available under some conditions.
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-30 , mycoplasma negative, HOECHST
Growth also as monolayer. Derived from hepatoma induced by N,N
Validated by: beuraldehyde dehydrogenase (high levels) * chromosome analysis (large metacentric chromosome 1)

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

Continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Contains inducible tyrosine aminotransferase.

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105