HTC's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HTC (rat, buffalo, ascitic fluid, hepatoma)

Monoclonal continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
Cell line available under some conditions.
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-30 , mycoplasma negative, HOECHST
Growth also as monolayer. Derived from hepatoma induced by N,N
Validated by: beuraldehyde dehydrogenase (high levels) * chromosome analysis (large metacentric chromosome 1)

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

ICLC catalog code: ATL95006
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone phosphate and hydrocortisone receptor

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium 70% + 20% Horse serum + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with MD, NP)

Availability in cell line catalogues: ECACC: 93120108

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci USA 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

Continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Contains inducible tyrosine aminotransferase.

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105