Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 35 years old)
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma
tumorigenic in nude mice
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * vesic. stomatitis (Indiana)
PROPERTIES: collagen prod.; chemotaxis; chemoinvasion; matrigel invasion; chemotherapeutic agents; cell biology; cytotoxicity; RNA studies; adhesion; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 121, ICLC: HTL98016, DSMZ: ACC 315
Bibliographic references:
- Cancer 1974;33:1027-1033 - PMID: 4132053
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 35 years old)
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma
tumorigenic in nude mice
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * vesic. stomatitis (Indiana)
PROPERTIES: collagen prod.; chemotaxis; chemoinvasion; matrigel invasion; chemotherapeutic agents; cell biology; cytotoxicity; RNA studies; adhesion; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 121, ICLC: HTL98016, DSMZ: ACC 315
Bibliographic references:
- Cancer 1974;33:1027-1033 - PMID: 4132053
ICLC catalog code: HTL98016
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 35 years old)
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * RNA tumor viruses * vesic. stomatitis (Indiana)
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 315
Bibliographic references:
- Cancer 1974;33:1027-1033 - PMID: 4132053
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 35 years old)
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma
tumorigenic in nude mice
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * vesic. stomatitis (Indiana)
PROPERTIES: collagen prod.; chemotaxis; chemoinvasion; matrigel invasion; chemotherapeutic agents; cell biology; cytotoxicity; RNA studies; adhesion; tumorigenicity; virology
Available in the following LABORATORY:
-
Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM + 10% FBS + 2mM L-Glutamine + non-essential amino acids; Freezing medium: Culture medium + 20% FBS + 5% DMSO; 37C, 5% CO2, split 6-8 Passage: 15 , mycoplasma negative
Validated by: isoenzymes (LDH, G6PD)
Availability in cell line catalogues: ATCC: CCL 121, ICLC: HTL98016, DSMZ: ACC 315
Bibliographic references:
- Cancer 1974;33:1027-1033 - PMID: 4132053
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 35 years old)
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma
tumorigenic in nude mice
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * vesic. stomatitis (Indiana)
PROPERTIES: collagen prod.; chemotaxis; chemoinvasion; matrigel invasion; chemotherapeutic agents; cell biology; cytotoxicity; RNA studies; adhesion; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 121, ICLC: HTL98016, DSMZ: ACC 315
Bibliographic references:
- Cancer 1974;33:1027-1033 - PMID: 4132053
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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