HEPA 1-6's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HEPA 1-6 (mouse, C57L, liver, hepatoma)

SPECIES: mouse, C57L
TISSUE/ORGAN: liver
TUMOR: hepatoma

ECACC catalog code: 92110305
Description: Mouse hepatoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Albumin, alpha-foetoprotein, alpha 1-antitrypsin, amylase

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1830, DSMZ: ACC 175, IZSBS: BS TCL 157

Bibliographic references:

J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
Methods Enzymol 1987;151:19-38 - PMID: 3431441

HEPA 1-6 (mouse, liver, hepatoma)

IZSBS catalog code: BS TCL 157
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: mouse
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: obtained from ATCC
PROPERTIES: secreting several liver-specific products (such as albumin, alpha-fetoprotein, alpha 1-antitrypsin and amylase)

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
GMEM + 10% FBS + 2mM L-Glutamine + 100 units/ml DIA/LIF + 0.1mM 2-mercaptoethanol; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CRL-1830, ECACC: 92110305, DSMZ: ACC 175

Bibliographic references:

J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
Methods Enzymol 1987;151:19-38 - PMID: 3431441

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105