Hepa 1-6's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Hepa 1-6 (mouse, C57L, liver, hepatoma)

SPECIES: mouse, C57L
TISSUE/ORGAN: liver
TUMOR: hepatoma

DSMZ catalog code: ACC 175
Morphology: adherent, epithelial cells growing as monolayer
Description: mouse hepatoma cell line was derived from the BW7756 tumor that arose in a C57L mouse; cells were described to secrete several liver-specific products (e.g. albumin, alpha1-antitrypsin, alpha-fetoprotein, amylase); cells may grow in serum-free medium when supplemented with dexamethasone and selenium (= ATCC CRL 1830) confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; cells grow rather quickly; seed out at ca. 1-2 x 106 cells/25 cm 2 at 37C with 5-10% CO2 about 10-15 x 106 cells/80 cm 2; doubling time of about 18 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype with centric fusion and 6% polyploidy; flat-moded, 53-70<4n>) * isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CRL 1830, ECACC: 92110305, IZSBS: BS TCL 157

Bibliographic references:

J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
Methods Enzymol 1987;151:19-38 - PMID: 3431441

Hepa 1-6 (mouse, liver, hepatoma)

IZSBS catalog code: BS TCL 157
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: mouse
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: obtained from ATCC
PROPERTIES: secreting several liver-specific products (such as albumin, alpha-fetoprotein, alpha 1-antitrypsin and amylase)

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
GMEM + 10% FBS + 2mM L-Glutamine + 100 units/ml DIA/LIF + 0.1mM 2-mercaptoethanol; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CRL-1830, ECACC: 92110305, DSMZ: ACC 175

Bibliographic references:

J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
Methods Enzymol 1987;151:19-38 - PMID: 3431441

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105