A-498's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A-498 (human, kidney, adenocarcinoma)

ICLC catalog code: HTL04005
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human (female, 52 years old)
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
DEPOSITOR: Dr. M. Viale, Pharmacology Dept, IST, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 three times a week using trypsin/EDTA (2-3 minutes); 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR

Availability in cell line catalogues: DSMZ: ACC 55, IZSBS: BS TCL 111

Bibliographic references:

J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

A-498 (human, kidney, carcinoma)

IZSBS catalog code: BS TCL 111
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 52 years old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: obtained from ATCC
PROPERTIES: expressing xenobiotic metabolizing enzymes

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 34 , mycoplasma negative, culture
Forming undifferentiated carcinomas in nude mice.
Validated by: isoenzymes (confirmed as human with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: HTB-44, ATCC: HTB-44, DSMZ: ACC 55, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-498 (human, kidney, carcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 52 years old)
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice

Available in the following LABORATORY:

Dip. Biochimica Biotecnologie Mediche, Universita' degli Studi (NAUBC, Napoli)
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: HTB 44, DSMZ: ACC 55, IZSBS: BS TCL 111, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105