SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
ECACC catalog code: 85011430
Morphology: Epithelial
Description: Human Caucasian hepatocyte carcinoma
Viruses: See Properties
Depositor: Prof B Knowles, Wistar Institute, Philadelphia, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Properties: Products: Prothrombin, antithrombin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen Applications: Study of hepatocyte function and specific protein expression
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 109;
Karyotype: Modal No 55
The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. Hep G2 cells have been shown to be G418 resistant (400µg/ml). The cells will respond to stimulation with human growth hormone.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
ICLC catalog code: HTL95005
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 15 years old)
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichimotrypsin, haptoglobin, ceruloplasmin, plasminogen, C3,C4, C3 act., fibrinogen, alpha1-acid glycop., alpha2-HS glycoprotein, beta lipoprotein and retinol binding protein; xenobiotic metabolism, drug metabolism assays, toxicity screening, hepatocyte studies
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-3 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 17; FGA: 22,25; TH01: 9; D18S51: 13,14; D21S11: 29,31; D8S1179: 15,16) * isoenzymes (confirmed as human with MD, MPI)
Availability in cell line catalogues: IZSBS: BS TCL79, ECACC: 85011430
Bibliographic references:
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
IZSBS catalog code: BS TCL79
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, ECACC: 85011430, ICLC: HTL95005
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Pharmacia Upjhon (MIECC, Nerviano (MI))
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: DMEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3 , mycoplasma negative
Epithelial-like morphology
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
MEM + 10% FBS + Antibiotics + 1% non-essential amino acids + 20mM Tricine + 1mM Na pyruvate; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-4
Validated by: biochemical assays * cellular assays
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
-
Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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