SPECIES: human, Black
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
ECACC catalog code: 87110901
Morphology: Epithelial
Susceptibility to viruses: adenovirus 5 * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human Negroid cervix carcinoma
Viruses: See Properties
Depositor: Mr C B Morris, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and virus studies: poliovirus 1 and Ad 3. Clonal analysis of mammalian cell populations
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Fetal Bovine Serum (FBS) or Hams F12 + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Modal No 68
Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmatio)
Availability in cell line catalogues: ATCC: CCL 2.2, ICLC: HTL95020
Bibliographic references:
ICLC catalog code: HTL95020
Continuous culture, grown as semiadherent
epithelial morphology
SPECIES: human, Black (female, 31 years old) Karyology: modal number 68
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
non tumorigenic
Susceptibility to viruses: adenovirus 5 * poliovirus 1 * vesic. stomatitis (Indiana)
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: toxicity screening; virology; bacterial adhesiveness; bacterial invasiveness; enzymology; tumorigenicity
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Semiadherent cell line, adaptable to growth in suspension (spinner) culture. Maintain culture between 3 and 9x105 cells/ml; split 1:2-1:4. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD, MD, NP, PepB)
Availability in cell line catalogues: ECACC: 87110901
Bibliographic references:
Monoclonal continuous culture, grown as suspension
epithelial morphology
SPECIES: human, Black (female, 31 years old) Karyology: modal number 68
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
non tumorigenic
Susceptibility to viruses: adenovirus 5 * poliovirus 1 * vesic. stomatitis (Indiana)
PROPERTIES: toxicity screening; virology; bacterial adhesiveness; bacterial invasiveness; enzymology; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 2.2, ECACC: 87110901, ICLC: HTL95020
Bibliographic references:
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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