A-431's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A-431 (human, carcinoma, epidermoid)

SPECIES: human
TUMOR: carcinoma, epidermoid

DSMZ catalog code: ACC 91
Morphology: epithelial-like, adherent cell line growing in monolayers
Description: human epidermoid carcinoma established from the solid tumor of an 85-year-old woman; cells were reported to have large numbers of EGF binding sites; cell line was used as indicator cell line for anti-TGF binding confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Welte, Medical School of Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures 1:4 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 7-12 x 106 cells/80 cm 2; doubling time of about 80-100 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with 24% polyploidy; 64(58-64)XX/XXX, -2, +3, +7, -8, +10, -11, -13, -14, -15, -16, -18, -22, +2 mar; del(3)(p11)x1-2, add(4)(p15), add(6)(p21.3), del(7)(p15), der(7)t(7;11)(p11/13;q11/13)add(11)(q23), add(8)( 21)) * cytogenetics 2 (add(11)(q23), der(14)t(13;14)(q11;p11), add(16)(p13); carries t(7;11) with breakpoint at EGFR (which it overexpresses); corresponds to published karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ATCC: CRL 1555

Bibliographic references:

Acta Paediatr 1992;81(4):287-291 - PMID: 1606385
Am J Pathol 1993;142(4):1141-1153 - PMID: 8475991
Am J Pathol 1993;143(4):1220-1225 - PMID: 7692735
Anal Biochem 1992;203(1):70-75 - PMID: 1524218
Anticancer Drugs 1992;3(3):245-252 - PMID: 1525405
Biochem Biophys Res Commun 1992;183(3):1216-1223 - PMID: 1533122
Biochem Biophys Res Commun 1992;187(1):152-157 - PMID: 1520296
Biochem J 1992;281(1):107-114 - PMID: 1370607
Biochemistry 1992;31(9):2459-2463 - PMID: 1547230
Cancer Res 1992;52(11):3189-3193 - PMID: 1591729
Cancer Res 1992;52(13):3515-3520 - PMID: 1319822
Cancer Res 1993;53(18):4322-4328 - PMID: 8364927
Exp Cell Res 1992;202(2):386-390 - PMID: 1397091
Exp Cell Res 1992;202(2):423-431 - PMID: 1327854
Invest Ophthalmol Vis Sci 1992;33(6):2053-2062 - PMID: 1582810
J Biochem 1992;112(2):386-390
J Biol Chem 1992;267(20):13943-13951 - PMID: 1629192
J Biol Chem 1992;267(20):14373-14381 - PMID: 1321146
J Biol Chem 1992;267(32):22860-22868 - PMID: 1429634
J Biol Chem 1992;267(32):23427-23433 - PMID: 1429683
J Biol Chem 1992;267(33):24034-24040 - PMID: 1429738
J Biol Chem 1992;267(35):25098-250103 - PMID: 1460012
J Biomater Sci Polym Ed 1993;4(3):235-343 - PMID: 8476793
J Cell Physiol 1992;150(1):180-187 - PMID: 1730782
J Cell Sci 1993;105(Pt1):219-231 - PMID: 8360275
J Dermatol 1992;19(2):78-81 - PMID: 1352313
J Histochem Cytochem 1993;41(2):313-317 - PMID: 8419466
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Virol 1992;66(1):197-203 - PMID: 727483
J Virol 1992;66(2):824-830 - PMID: 1309916
Mol Cell Biol 1992;12(12):5816-5823 - PMID: 1333046
Mol Cell Biol 1992;12(12):5843-5856 - PMID: 1448108
Oncogene 1993;8(10):2857-2862 - PMID: 8397371
Oncogene 1993;8(10):3141-3147 - PMID: 8414516
Oncol Res 1992;4(4-5):193-200 - PMID: 1324032
Recent Prog Horm Res 1993;48:511-516 - PMID: 8441859

A-431 (human, skin, carcinoma, squamous cell)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 85 years old)
TISSUE/ORGAN: skin
TUMOR: carcinoma, squamous cell
PROPERTIES: EGF receptor expression; growth factors; cell cycle

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5 Passage: 40

Availability in cell line catalogues: ATCC: CRL 1555, DSMZ: ACC 91

Bibliographic references:

Acta Paediatr 1992;81(4):287-291 - PMID: 1606385
Am J Pathol 1993;142(4):1141-1153 - PMID: 8475991
Am J Pathol 1993;143(4):1220-1225 - PMID: 7692735
Anal Biochem 1992;203(1):70-75 - PMID: 1524218
Anticancer Drugs 1992;3(3):245-252 - PMID: 1525405
Biochem Biophys Res Commun 1992;183(3):1216-1223 - PMID: 1533122
Biochem Biophys Res Commun 1992;187(1):152-157 - PMID: 1520296
Biochem J 1992;281(1):107-114 - PMID: 1370607
Biochemistry 1992;31(9):2459-2463 - PMID: 1547230
Cancer Res 1992;52(11):3189-3193 - PMID: 1591729
Cancer Res 1992;52(13):3515-3520 - PMID: 1319822
Cancer Res 1993;53(18):4322-4328 - PMID: 8364927
Exp Cell Res 1992;202(2):386-390 - PMID: 1397091
Exp Cell Res 1992;202(2):423-431 - PMID: 1327854
Invest Ophthalmol Vis Sci 1992;33(6):2053-2062 - PMID: 1582810
J Biochem 1992;112(2):386-390
J Biol Chem 1992;267(20):13943-13951 - PMID: 1629192
J Biol Chem 1992;267(20):14373-14381 - PMID: 1321146
J Biol Chem 1992;267(32):22860-22868 - PMID: 1429634
J Biol Chem 1992;267(32):23427-23433 - PMID: 1429683
J Biol Chem 1992;267(33):24034-24040 - PMID: 1429738
J Biol Chem 1992;267(35):25098-250103 - PMID: 1460012
J Biomater Sci Polym Ed 1993;4(3):235-343 - PMID: 8476793
J Cell Physiol 1992;150(1):180-187 - PMID: 1730782
J Cell Sci 1993;105(Pt1):219-231 - PMID: 8360275
J Dermatol 1992;19(2):78-81 - PMID: 1352313
J Histochem Cytochem 1993;41(2):313-317 - PMID: 8419466
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Virol 1992;66(1):197-203 - PMID: 727483
J Virol 1992;66(2):824-830 - PMID: 1309916
Mol Cell Biol 1992;12(12):5816-5823 - PMID: 1333046
Mol Cell Biol 1992;12(12):5843-5856 - PMID: 1448108
Oncogene 1993;8(10):2857-2862 - PMID: 8397371
Oncogene 1993;8(10):3141-3147 - PMID: 8414516
Oncol Res 1992;4(4-5):193-200 - PMID: 1324032
Recent Prog Horm Res 1993;48:511-516 - PMID: 8441859

A-431 (human, skin, carcinoma, squamous cell)

Available in the following LABORATORY:

Universita' degli Studi (NAUMC, Napoli)
MEM + 10% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: MEM + 10% FBS + 10% DMSO; 37C, 5% CO2, split 3-4.
EGF receptor highly expressed.

Availability in cell line catalogues: ATCC: CRL 1555, DSMZ: ACC 91

Bibliographic references:

Acta Paediatr 1992;81(4):287-291 - PMID: 1606385
Am J Pathol 1993;142(4):1141-1153 - PMID: 8475991
Am J Pathol 1993;143(4):1220-1225 - PMID: 7692735
Anal Biochem 1992;203(1):70-75 - PMID: 1524218
Anticancer Drugs 1992;3(3):245-252 - PMID: 1525405
Biochem Biophys Res Commun 1992;183(3):1216-1223 - PMID: 1533122
Biochem Biophys Res Commun 1992;187(1):152-157 - PMID: 1520296
Biochem J 1992;281(1):107-114 - PMID: 1370607
Biochemistry 1992;31(9):2459-2463 - PMID: 1547230
Cancer Res 1992;52(11):3189-3193 - PMID: 1591729
Cancer Res 1992;52(13):3515-3520 - PMID: 1319822
Cancer Res 1993;53(18):4322-4328 - PMID: 8364927
Exp Cell Res 1992;202(2):386-390 - PMID: 1397091
Exp Cell Res 1992;202(2):423-431 - PMID: 1327854
Invest Ophthalmol Vis Sci 1992;33(6):2053-2062 - PMID: 1582810
J Biochem 1992;112(2):386-390
J Biol Chem 1992;267(20):13943-13951 - PMID: 1629192
J Biol Chem 1992;267(20):14373-14381 - PMID: 1321146
J Biol Chem 1992;267(32):22860-22868 - PMID: 1429634
J Biol Chem 1992;267(32):23427-23433 - PMID: 1429683
J Biol Chem 1992;267(33):24034-24040 - PMID: 1429738
J Biol Chem 1992;267(35):25098-250103 - PMID: 1460012
J Biomater Sci Polym Ed 1993;4(3):235-343 - PMID: 8476793
J Cell Physiol 1992;150(1):180-187 - PMID: 1730782
J Cell Sci 1993;105(Pt1):219-231 - PMID: 8360275
J Dermatol 1992;19(2):78-81 - PMID: 1352313
J Histochem Cytochem 1993;41(2):313-317 - PMID: 8419466
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Virol 1992;66(1):197-203 - PMID: 727483
J Virol 1992;66(2):824-830 - PMID: 1309916
Mol Cell Biol 1992;12(12):5816-5823 - PMID: 1333046
Mol Cell Biol 1992;12(12):5843-5856 - PMID: 1448108
Oncogene 1993;8(10):2857-2862 - PMID: 8397371
Oncogene 1993;8(10):3141-3147 - PMID: 8414516
Oncol Res 1992;4(4-5):193-200 - PMID: 1324032
Recent Prog Horm Res 1993;48:511-516 - PMID: 8441859

A-431 (human, skin, carcinoma, squamous cell)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CRL 1555, DSMZ: ACC 91

Bibliographic references:

Acta Paediatr 1992;81(4):287-291 - PMID: 1606385
Am J Pathol 1993;142(4):1141-1153 - PMID: 8475991
Am J Pathol 1993;143(4):1220-1225 - PMID: 7692735
Anal Biochem 1992;203(1):70-75 - PMID: 1524218
Anticancer Drugs 1992;3(3):245-252 - PMID: 1525405
Biochem Biophys Res Commun 1992;183(3):1216-1223 - PMID: 1533122
Biochem Biophys Res Commun 1992;187(1):152-157 - PMID: 1520296
Biochem J 1992;281(1):107-114 - PMID: 1370607
Biochemistry 1992;31(9):2459-2463 - PMID: 1547230
Cancer Res 1992;52(11):3189-3193 - PMID: 1591729
Cancer Res 1992;52(13):3515-3520 - PMID: 1319822
Cancer Res 1993;53(18):4322-4328 - PMID: 8364927
Exp Cell Res 1992;202(2):386-390 - PMID: 1397091
Exp Cell Res 1992;202(2):423-431 - PMID: 1327854
Invest Ophthalmol Vis Sci 1992;33(6):2053-2062 - PMID: 1582810
J Biochem 1992;112(2):386-390
J Biol Chem 1992;267(20):13943-13951 - PMID: 1629192
J Biol Chem 1992;267(20):14373-14381 - PMID: 1321146
J Biol Chem 1992;267(32):22860-22868 - PMID: 1429634
J Biol Chem 1992;267(32):23427-23433 - PMID: 1429683
J Biol Chem 1992;267(33):24034-24040 - PMID: 1429738
J Biol Chem 1992;267(35):25098-250103 - PMID: 1460012
J Biomater Sci Polym Ed 1993;4(3):235-343 - PMID: 8476793
J Cell Physiol 1992;150(1):180-187 - PMID: 1730782
J Cell Sci 1993;105(Pt1):219-231 - PMID: 8360275
J Dermatol 1992;19(2):78-81 - PMID: 1352313
J Histochem Cytochem 1993;41(2):313-317 - PMID: 8419466
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
J Virol 1992;66(1):197-203 - PMID: 727483
J Virol 1992;66(2):824-830 - PMID: 1309916
Mol Cell Biol 1992;12(12):5816-5823 - PMID: 1333046
Mol Cell Biol 1992;12(12):5843-5856 - PMID: 1448108
Oncogene 1993;8(10):2857-2862 - PMID: 8397371
Oncogene 1993;8(10):3141-3147 - PMID: 8414516
Oncol Res 1992;4(4-5):193-200 - PMID: 1324032
Recent Prog Horm Res 1993;48:511-516 - PMID: 8441859

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105