HEL 299's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HEL 299 (human, Black, lung, embryonic)

SPECIES: human, Black
TISSUE/ORGAN: lung, embryonic

ECACC catalog code: 87042207
Morphology: Fibroblast
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human Negroid embryo lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Enzymatic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Limited lifespan.
Passages: 19; Karyotype: 2n = 46, diploid
Derived from embryonic lung tissue of a Negro male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 137, IZSBS: BS CL49

Bibliographic references:

Arch Virol 1982;72:127 - PMID: 6285862
Proc Soc Exp Biol Med 1968;128:772 - PMID: 5668122

HEL 299 (human, Caucasian, lung, embryonic)

IZSBS catalog code: BS CL49
Grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
PROPERTIES: biochemistry; cell biology; genetics; enzymology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 18 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 137, ECACC: 87042207

HEL 299 (human, Caucasian, lung, embryonic)

Grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
PROPERTIES: biochemistry; cell biology; genetics; enzymology

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2, split 1-2 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 137, IZSBS: BS CL49, ECACC: 87042207

Bibliographic references:

Arch Virol 1982;72:127 - PMID: 6285862
Proc Soc Exp Biol Med 1968;128:772 - PMID: 5668122

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105