HCT-8's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HCT-8 (human, intestine, ileocecal, adenocarcinoma)

SPECIES: human
TISSUE/ORGAN: intestine, ileocecal
TUMOR: adenocarcinoma

ECACC catalog code: 90032006
Morphology: Epithelial
Description: Human ileocecal adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 25; Karyotype: 2n = 46, pseudodiploid
From 67 year old male. Produce CEA and are considered identical to HRT-18.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 244, ICLC: HTL99005, IZSBS: BS TCL 97

Bibliographic references:

Int J Cancer 1975;16:74 - PMID: 1058173
J Natl Cancer Inst 1974;52:1101-1110 - PMID: 4826581

HCT-8 (human, intestine, ileocecal, adenocarcinoma)

ICLC catalog code: HTL99005
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 67 years old)
TISSUE/ORGAN: intestine, ileocecal
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Dr A. Sobrero, Udine, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production; the cell line was demonstrated by cytogenetic analysis to be identical to HRT-18 cell line

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% HS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: ECACC: 90032006, IZSBS: BS TCL 97

Bibliographic references:

Int J Cancer 1975;16:74-82
J Natl Cancer Inst 1974;52:1101-1110

HCT-8 (human, intestine, ileocecal, adenocarcinoma)

IZSBS catalog code: BS TCL 97
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human (male, 67-year-old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: intestine, ileocecal
TUMOR: adenocarcinoma
DEPOSITOR: obtained from ATCC
PROPERTIES: production of carcinoembryonic antigen

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% HS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, PEPB)

Availability in cell line catalogues: ATCC: CCL-244, ECACC: 90032006, ICLC: HTL99005

Bibliographic references:

J Natl Cancer Inst 1974;52:1101-1110 - PMID: 4826581

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105