Hak's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Hak (hamster, Syrian, kidney)

SPECIES: hamster, Syrian
TISSUE/ORGAN: kidney

ECACC catalog code: 90102522
Morphology: Small, Epithelial-like
Susceptibility to viruses: alpha viruses * arbovirus * Asian strain influenza * Coxsackie A * Coxsackie A8 * Coxsackie B1 * herpes simplex * influenza * smallpox * vesic. stomatitis (Indiana)
Description: Hamster Syrian kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Arboviruses, Coxsackie A4, A8, B1, HSV, smallpox influenza virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Cells will grow in a piled up manner.
Passages: 102; Karyotype: Modal No 56
From 2 normal young adult males. Capable of growth in suspension (spinner) cultures. Useful for growth of several viruses including arboviruses, Coxsackie A4, A8, B1, HSV, smallpox and Asian strain influenza.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 15, IZSBS: BS CL48

Bibliographic references:

S Afr J Med Sci 1963;28:81

Hak (hamster, Syrian, kidney)

IZSBS catalog code: BS CL48
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: hamster, Syrian (male)
TISSUE/ORGAN: kidney
Susceptibility to viruses: alpha viruses * arbovirus * Asian strain influenza * Coxsackie A * Coxsackie A8 * Coxsackie B1 * herpes simplex * influenza * smallpox * vesic. stomatitis (Indiana)
PROPERTIES: virology; virus replication

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
BME (HBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 109 , mycoplasma negative, culture
Cell line can also grow in suspension.

Availability in cell line catalogues: ATCC: CCL 15, ECACC: 90102522

Hak (hamster, Syrian, kidney)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: hamster, Syrian (male)
TISSUE/ORGAN: kidney
Susceptibility to viruses: alpha viruses * arbovirus * Asian strain influenza * Coxsackie A * Coxsackie A8 * Coxsackie B1 * herpes simplex * influenza * smallpox * vesic. stomatitis (Indiana)
PROPERTIES: virology; virus replication

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 15, IZSBS: BS CL48, ECACC: 90102522

Bibliographic references:

S Afr J Med Sci 1963;28:81

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105