GIRARDI HEART's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

GIRARDI HEART (human, heart)

IZSBS catalog code: BS CL 47
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 41 years old) Karyology: 2n = 45 in 53 cells
TISSUE/ORGAN: heart
Susceptibility to viruses: adenovirus 3 * poliovirus 1 * poliovirus 3 * vesic. stomatitis (Indiana)
DEPOSITOR: obtained from ATCC
PROPERTIES: virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 509 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, AST, LD)

Availability in cell line catalogues: ECACC: 93120822

Bibliographic references:

Fed Proc 1975;16:14

GIRARDI HEART (human, heart)

Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human (male, 41 years old)
TISSUE/ORGAN: heart
Susceptibility to viruses: adenovirus 3 * adenovirus 4 * adenovirus 7 * canine distemper virus * Coxsackie A11 * Coxsackie A13 * Coxsackie A15 * Coxsackie A18 * Coxsackie B * echovirus * ECM * herpes * measles * mumps * Newcastle disease virus * parainfluenza 2 * poliovirus 1 * poliovirus 3 * RS * vesic. stomatitis (Indiana)
PROPERTIES: biochemistry; tumorigenicity; virus titration; cell biology; CF antigen prod.; virology

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
The cells not contain HSL antigen and derived from heart (right atrial appendage). Tumour induced in the cheek pouch of the Syrian hamster.

Availability in cell line catalogues: ATCC: CCL 47, IZSBS: BS CL 47, ECACC: 93120822

Bibliographic references:

Fed Proc 1975;16:414
J Natl Cancer Inst 1964;33:285
Proc Soc Exp Biol Med 1958;98:18

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105