GH3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

GH3 (rat, Wistar-Furth, pituitary, pituitary tumor)

SPECIES: rat, Wistar-Furth
TISSUE/ORGAN: pituitary
TUMOR: pituitary tumor

ECACC catalog code: 87012603
Morphology: Epithelial
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: Rat Wistar-Furth pituitary tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Growth hormone, prolactin Applications: Hormone studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Upon thawing, time before the 1st subculture can be performed may be extended (10-15 days).
Passages: 35; Karyotype: Hyperdiploid, Modal No 60
Derived from a 7 month female Wistar-Furth rat. Cells produce growth hormone faster than GH1amd also produces prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 82.1, ICLC: ATL96003

Bibliographic references:

Endocrinology 1968;82:342 - PMID: 4951281
In Vitro 1970;6:180 - PMID: 5535574
J Cell Biol 1969;43:432 - PMID: 5389137

GH3 (rat, Wistar-Furth, pituitary tumor)

ICLC catalog code: ATL96003
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar-Furth (female, 7 months old)
TUMOR: pituitary tumor
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Growth hormone, somatotropin and prolactin secretion.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F10 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; the cells are capable of adaptation in suspension culture using MEM. Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with MD, MPI)

Availability in cell line catalogues: ECACC: 87012603

Bibliographic references:

Endocrinology 1968;82:342 - PMID: 4951281
In Vitro 1970;6:180 - PMID: 5535574
J Cell Biol 1969;43:432 - PMID: 5389137

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105