TISSUE/ORGAN: heart, embryonic
ECACC catalog code: 87043002
Description: Bovine embryo heart
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 100-200ng/ml/day Fibroblast Growth Factor (FGF) + 10% Fetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hrs and wash with PBS. Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2 37C.
The cells have an absolute dependency on fibroblast growth factor for survival. The cells are BVDV negative. Fresh Fibroblast growth factor (FGF) should be added daily.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1395, IZSBS: BS CL113
- Proc Natl Acad Sci U S A 1976;73:4120 - PMID: 1069301
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.