EL4's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

EL4 (mouse, blood, lymphoma)

SPECIES: mouse
TISSUE/ORGAN: blood
TUMOR: lymphoma

ECACC catalog code: 85023105
Morphology: Lymphoblast
Description: Mouse ascites lymphoma lymphoblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2 Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 39
Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol (10e4M) and dexamethasone and is sensitive to PHA.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB-39

Bibliographic references:

Br J Cancer 1950;4:372
Cancer Res 1965;25:813 - PMID: 4284252
J Natl Cancer Inst 1972;48:265 - PMID: 4119883

EL4 (mouse, C57BL/6N, ascitic fluid, lymphoma, lymphoblastic)

Continuous culture, grown as suspension
lymphoid morphology
SPECIES: mouse, C57BL/6N
TISSUE/ORGAN: ascitic fluid
TUMOR: lymphoma, lymphoblastic
PROPERTIES: interferon prod.; IL-1 prod.; resistant to cortisol and dexamethasone; tumorigenicity; virology

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2,split 1-2

Availability in cell line catalogues: ATCC: TIB-39, ECACC: 85023105

Bibliographic references:

Br J Cancer 1950;4:372
Cancer Res 1965;25:813 - PMID: 4284252
J Natl Cancer Inst 1972;48:265 - PMID: 4119883

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105