ECV304's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

ECV304 (human, Asiatic, umbilical cord)

IZSBS catalog code: BS CL 137
Continuous culture, grown as monolayer
Endothelial-like morphology
SPECIES: human, Asiatic (female, new born) Karyology: high trisomic
TISSUE/ORGAN: umbilical cord
spontaneously trasformed
tumorigenic in athymic nude mice
Susceptibility to viruses: Semlik Forest virus
DEPOSITOR: obtained from ATCC
PROPERTIES: contact inhibition; IL-6 prod. after stimulation with IL-1

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
Medium 199 (EBSS) + 10% FBS (Heat Inactivated); Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 128 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, LD)

Availability in cell line catalogues: ATCC: CRL-1998, ECACC: 92091712

Bibliographic references:

In Vitro Cell Dev Biol 1990;26:265 - PMID: 1690702
In Vitro Cell Dev Biol 1991;27A:766 - PMID: 1960144
In Vitro Cell Dev Biol 1992;28A:380 - PMID: 1378828

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105