6-23 (clone 6)'s homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

6-23 (clone 6) (rat, thyroid, medullary, carcinoma)

IZSBS catalog code: TCL 130
Continuous culture, grown as monolayer
Fibroblast-like morphology
SPECIES: rat Karyology: unknown
TISSUE/ORGAN: thyroid, medullary
TUMOR: carcinoma
DEPOSITOR: obtained from ATCC
PROPERTIES: secreting peptides (neurotensin and calcitonin)

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
DMEM (4.5 g/l glucose) + 10% HS ; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
It is of C-cell origin derived from the non-clonal line rMTC 6-23; producing neurotensin and calcitonin after stimulation by calcium, KCl, glucagon and catecholamines. Cell adhesion must be improved by using flasks coated with collagen type IV.

Availability in cell line catalogues: ATCC: CRL-1607, ECACC: 87042206

Bibliographic references:

Endocrinology 1980;107:509-516 - PMID: 6248326
Proc Natl Acad Sci U S A 1980;77:3741-3745 - PMID: 6932046

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105