DU-145's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

DU-145 (human, Caucasian, prostate, carcinoma)

ICLC catalog code: HTL99013
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 69 years old)
TISSUE/ORGAN: prostate
TUMOR: carcinoma
DEPOSITOR: Dr P. Limonta, Institute of Endocrinology, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 5-10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 Passage: 67 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 17,18; FGA: 22; TH01: 7; D18S51: 12; D21S11: 29,30,33,71; D8S1179: 13,14)

Availability in cell line catalogues: DSMZ: ACC 261

Bibliographic references:

Cancer Res 1977, 37:4049-4058 - PMID: 908039
Int J Cancer 1978, 21:274-281 - PMID: 631930

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105