SPECIES: dog, golden retriever
ECACC catalog code: 94062922
Description: Canine Monocyte-macrophage
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 5,192,679
Properties: Receptors: FC-gamma receptors
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS) (Heat Inactivated). Maintain cultures at 3-9x100,000 cells/ml; 5% CO2; 37C.
Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 10389, ICLC: ATL96008
- In Vitro Cell Dev Biol 1988;24:223-229 - PMID: 3350786
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.