5637's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

5637 (guinea pig, 2/N, fetal)

SPECIES: guinea pig, 2/N
TISSUE/ORGAN: fetal

ECACC catalog code: 90110524
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Guinea pig fetal cells transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 32; Karyotype: Diploid
Derived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1405, DSMZ: ACC 35, ICLC: HTL01017

Bibliographic references:

Cancer Res 1975;35:1035 - PMID: 1116142
Cancer Res 1975;35:1045 - PMID: 1116143
Cancer Res 1976;36:128 - PMID: 1247991

5637 (human, Caucasian, bladder, carcinoma)

ICLC catalog code: HTL01017
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 68 year old)
TISSUE/ORGAN: bladder
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: Dr M. Musso, Oncology, Biology and Genetic Dept, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: GM-CSF, IL-1, IL-6 production

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:8 using trypsin/EDTA (8-10 min.); 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 18; FGA: 22; TH01: 7,9; D18S51: --; D21S11: 36; D8S1179: 10,16)

Availability in cell line catalogues: ECACC: 90110524, DSMZ: ACC 35

Bibliographic references:

J Natl Cancer Inst 1977;59:221-226 - PMID: 77210034
Natl Cancer Inst Monogr 1978;49:5-9 - PMID: 79156922

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105