SPECIES: human, Caucasian
TISSUE/ORGAN: skin, embryonic
ECACC catalog code: 86082802
Susceptibility to viruses: herpes * poliovirus 1 * SV40 * vesic. stomatitis (Indiana)
Description: Human Caucasian embryo skin
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome and virus studies (Normal control): herpesviruses, SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Finite lifespan; at most 25 subcultures from original tissue.
Karyotype: 2n = 46
Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 110, IZSBS: BS CL34
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.