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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Detroit 539 (human, Caucasian, skin, fibroblast, trisomy 21 / Down syndrome)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Luteinising Hormone (LH) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. A further 15-20 passages expected.
  Karyotype: Aneuploid
  Derived from a female caucasian infant with Down Syndrome. Karyotypic analysis shows 90% cell population possesses 47 chromosomes with 5 chromosomes belonging to G group. These findings persist essentially unchanged throughout at least 30 serial subcultures.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 84, IZSBS: BS CL31